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Radioprotective effect of propolis and quercetin gamma-irradiated mice evaluated by the alkaline comet assay


Benković, Vesna; Horvat Knežević, Anica; Đikić, Domagoj; Lisičić, Duje; Oršolić, Nada; Bašić, Ivan; Kosalec, Ivan; Kopjar, Nevenka
Radioprotective effect of propolis and quercetin gamma-irradiated mice evaluated by the alkaline comet assay // Phytomedicine, 15 (2008), 10; 851-858 doi:10.1016/j.phymed.2008.02.010 (međunarodna recenzija, članak, znanstveni)


Naslov
Radioprotective effect of propolis and quercetin gamma-irradiated mice evaluated by the alkaline comet assay

Autori
Benković, Vesna ; Horvat Knežević, Anica ; Đikić, Domagoj ; Lisičić, Duje ; Oršolić, Nada ; Bašić, Ivan ; Kosalec, Ivan ; Kopjar, Nevenka

Izvornik
Phytomedicine (0944-7113) 15 (2008), 10; 851-858

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Alkaline comet assay; white blood cells; mice; propolis; quercetin; radioprotection

Sažetak
The radioprotective effects of ethanolic extract of propolis (EEP) and quercetin on the white blood cells of the whole-body irradiated CBA mice were investigated. Irradiation was performed using a γ -ray source (60Co), and absorbed dose was 9 Gy. The efficiency of test components was evaluated when given intraperitoneally (ip) at dose of 100 mg kg-1 for 3 consecutive days before and/or after irradiation. Moreover, possible genotoxic effects of test components were also assessed on non-irradiated animals. For each experimental group leukocyte count was determined and the primary DNA damage in leukocytes was assessed using the alkaline comet assay. The higher efficiency of EEP and quercetin was observed when given preventively. The results suggest that propolis and quercetin given to mice before irradiation protect their white blood cells from lethal effects of irradiation and diminish primary DNA damage as confirmed by the alkaline comet assay. Positive results obtained on gamma-irradiated mice given EEP and quercetin, complementary with our earlier observations on survival of irradiated mice ; indicate that these compounds could be considered effective non-toxic radioprotectors. The exact mechanisms of radioprotection by these compounds and their effects on DNA repair processes still are to be elucidated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
006-0061117-1242 - Mikromicete, interakcije toksičnih metabolita-zdravlje i prevencija (Maja Šegvić Klarić, )
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
119-0000000-1255 - Kemoprevencija rasta tumora polifenolnim sastavnicama (Nada Oršolić, )
119-0532265-1254 - Imunoadjuvantno i zaštitno djelovanje propolisa u životinja (Vesna Benković, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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