engleski

DIFFERENT APPROACHES FOR DNA ANALYSIS OF OLD BONE SAMPLES – PRELIMINARY RESULTS

The process of identifying mortal remains from different mass fatalities has advanced significantly in last decade. However, obtaining DNA profiles from skeletal remains is among the most challenging types of DNA forensic testing. The development of STR profiles from bone or tooth samples is not without its limitation. While working with few decades old bones and teeth forensic scientists are usually confronted by many problems (insufficient quantity of DNA, high level of DNA degradation, presence of PCR inhibitors etc.). Each laboratory may encounter significant difficulties when developing their own procedures for this kind of testing. This presentation is based on preliminary and brief comparison of different approaches in three crucial stages of DNA analysis of skeletal remains: bone powdering procedure, DNA isolation and amplification protocols. We have briefly analyzed two different approaches of bone powdering (blender vs. Dremel tool), DNA isolation procedures (phenol-chlorophorm vs. Optimized Qiagen procedure) and PCR protocols for PowerPlex16 kit (regular vs. extended). Earlier processed and profiled 60 years old bone samples were used in this analysis. QuantifilerTM Human DNA Quantification Kit was used for DNA quantification and electrophoresis of the amplification products was performed on ABI PRISM 310 genetic analyzer. Obtained results indicate advantages but also some disadvantages of all examined methods.

DNA analysis; old bone samples; bonepowdering; DNA extraction; PCR protocol

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