engleski

ANALYSIS OF TWO DIFFERENT METHODS IN DNA EXTRACTION FROM SALIVA SPECIMEN

Saliva is recognized as suitable source of genomic DNA for various genetic studies. That is the sample, which could be relatively easily collected in a painless and non-invasive manner. Also, when is collected, it could be successfully stored through extended period of time. The aim of this work was to observe possibility of application of two different approaches in DNA extraction from saliva specimens for DNA forensic analysis purposes. One is usage of commercial Qiagen protocol for DNA extraction from buccal swab, semen and blood, on saliva specimens collected with passive oral lavage in micro tubes and another-one is application of the Oragen TM DNA Self – Collection Kit. Of laboratory protocol for manual extraction of DNA from 0, 5 ml Oragen TM /saliva solution. The samples of saliva were collected from the volunteers. Promega PowerPlex 16 kit was employed for simultaneous amplification of 15 STR loci in PE GeneAmp PCR Thermocycler 9700. ABI PRISM 377 Sequencer was used in analysis of the amplified fragments. Given results confirmed the successfulness of application of standard Qiagen protocol for DNA extraction from saliva without need for its additional modification in order to get an optimal amount of DNA for further phases of forensic DNA analysis. Furthermore, it was pointed that Oragen approach could be also used in the same purpose, but with supplementary optimisation of isolation protocol.

collecting sample; saliva specimens; DNA extraction; different methods; DNA analysis

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