Allele frequencies for the fifteen short tandem repeat loci in Croatian population (CROSBI ID 537375)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Škaro, Vedrana ; Projić, Petar ; Pojskic, Naris ; Adaleta, Durmić ; Kovačević, Lejla ; Haverić, Sanin ; Primorac, Dragan ; Marjanović, Damir
engleski
Allele frequencies for the fifteen short tandem repeat loci in Croatian population
We have analyzed the distribution of allele frequencies at fifteen autosomal short tandem repeats loci in the representative sample of Croatians. A total of 110 unrelated individuals (Caucasians) born in Croatia have been sampled for the analysis. All of them have been voluntary donors. Buccal swab have been used as the DNA source. Specimens were air-dried, placed in 1, 5 ml tubes, and immediately transported to the laboratory. The samples have been stored at -20oC until beginning of DNA analysis. The Qiagen DnaeasyTM Tissue Kit was used for DNA extraction. The AmpFlSTR® ; ; ; Identifiler® ; ; ; (ABI, Foster City, CA) has been used to simultaneously amplify by PCR 15 STR loci. The STR loci are: D3S1358, TH01, D21S11, D18S51, D2S1338, D5S818, D13S317, D7S820, D16S539, CSF1PO, D19S433, vWA, D8S1179, TPOX, and FGA. Similar amount of DNA (approx. 1 ng) was used in all PCR reactions. Total reaction volume was 12, 5 μ l. The PCR amplification has been carried out in PE Gene Amp PCR System Thermal Cycler (ABI, Foster City, CA) according to the manufacturer’ s recommendations. Electrophoresis of the amplification products was preformed on an ABI PRISM 3130 genetic analyzer Raw data have been compiled, analyzed and numerical allele designations of the profiles were obtained by using the accessory software: ABI PRISM® ; ; ; Data Collection Software v3.0 and GeneMapperΤ Μ ID Software v3.1. Deviation from Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination and power of exclusion were calculated. Also, we have compared our data with data obtained from geographically neighboring European populations.
DNA typing ; short tandem repeats ; Croatian population
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Podaci o prilogu
289-289.
2007.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
European journal of human genetics
Nature publishing group
1018-4813
1476-5438
Podaci o skupu
European Human Genetics Conference
poster
16.06.2007-19.06.2007
Nica, Francuska