engleski

Population study at two additional STR loci D2S1338 and D19S433 in the representative sample of multinational Bosnia and Herzegovina residents

In our previous population studies of B&H human population, we have used 15 STR loci included in the PowerPlex 16&reg ; ; ; System, twelve Y-chromosomal short tandem repeats loci incorporated in the PowerPlex&reg ; ; ; Y System and 28 Y-chromosome NRY bi-allelic markers. All obtained results were included in Bosnian referent database. In order of future development of this database we have decide to analyze two additional STR loci: D2S1338 and D19S433. Therefore, we have tested 110 unrelated healthy individuals born in the Bosnia and Herzegovina, from three main ethnical groups. Qiagen DnaeasyTM Tissue Kit was used for DNA extraction from buccal swabs and blood stains, QuantiBlot&reg ; ; ; assay for quantification and AmpFlSTR&reg ; ; ; Identifiler&reg ; ; ; for amplification and detection. Amplification was carried out as described previously. The total volume of each reaction was 12.5μ l. The PCR amplifications have been carried out in PE GeneAmp PCR-System 9700 Thermal Cycler according to the manufacturer’ s recommendations. Electrophoresis of the amplification products was preformed on an ABI PRISM 3100. Numerical allele designations of the profiles were obtained by processing with GeneScan&reg ; ; ; 3.7.1 and Genotyper&reg ; ; ; 3.7. Deviation from Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination and exclusion were calculated. Also, we have compared B&H data with data obtained from geographically closer (neighboring) European populations. Results of this study are going to be used as guidelines in additional investigation of genetic relationship between recent B&H and neighboring populations, originated in our previous studies on Y-chromosome bi-allelic markers, as well in its application in the field of population, human and forensic genetics.

D2S1338; D19S433; population data

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