engleski

Semithin sections of epoxy resin embedded mouse embryos in morphological analysis of whole mount in situ RNA hybridization

Descriptive morphological studies are often combined with gene expression pattern analyses. Unembedded vibratome or cryotome sections are compatible with in situ RNA hybridization, but spatial resolution is rather low for precise microscopic studies necessary in embryology. Therefore, use of plastic embedding media, which allow semithin and ultrathin sectioning for light and electron microscopy, could be an important advantage. This work suggested a new approach based on the whole mount hybridisation of mouse embryos and subsequent epoxy resin embedding. Epoxy resin allowed serial sectioning of semithin sections with preserved in situ RNA hybridization signal, which was a necessary prerequisite for precise morphological analysis of embryo development.

in situ hibridization; gene expression; semi-thin sections; embryo; mouse

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