engleski

Regulation of Osteopontin Expression in Endometrial and Decidual cells

The steroid hormone progesterone plays a central role in regulation of cell proliferation and differentiation that control implantation and decidualization. The progesterone responsiveness is determined by the extent of expression of specific progesterone receptors which mediate progesterone action via genomic and non-genomic pathways. In human, rodents, and many other species the genomic actions of progesterone are mediated by intracellular receptors, progesterone receptor A (PR-A) and progesterone receptor B (PR-B), which are members of the nuclear receptor superfamily of transcription factors. Implantation and decidualisation is impaired in the genetically modified mice missing either PR or PR-A isoform, and result is infertility. Implantation and decidualisation involves pregnancy specific remodeling of extracellular matrix (ECM) at the fetal maternal interface. Gene profiling using microarray analysis of endometrial receptivity identified osteopontin (secreted phosphoprotein 1) as the most differentially regulated ECM-adhesion molecule in the human uterus as it becomes receptive to implantation. Osteopontin is a chemokine-like ECM associated glycosylated phosphoprotein which binds to integrin heterodimers via its RGD sequence to promote cell adhesion and migration. Objective of this study is to analyze the role of the progesterone receptors in the regulation of osteopontin expression in the stromal endometrium and decidual cells. We found that expression of endometrial osteoponrtin is estrogen and progesterone regulated.

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