engleski

Burn-related expansion of perforin-expressing lymphocytes in peripheral blood

Cytotoxic T cells (CTL) and natural killer (NK) cells induce cell death of their target cells either by the release of perforin and granzymes or by the surface interaction between Fas ligand and Fas. Both these lytic pathways induce apoptosis of neoplastic and an altered-self cell. The mean idea of this study was to examine alteration of cell mediate immunity and cytolytic molecule perforin in different lymphocyte subpopulation in peripheral blood of burned patients. Venous blood samples were taken from all examinees and peripheral blood leukocytes were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometry (FACSCalibur). Statistical analysis was done in computer program Statistica 7.1 (StatSoft, Inc., Tulsa, OK, USA). Graphs and tables were done in computer program Microsoft Excel. The results demonstrated that only 10-15% of PBL in patients are perforin positive (total perforin of burned patients are 40% lower than in control group). Percentage of all double positive cells showed significant decrease (CD3+ P+ , CD4+P+, CD8+P+, CD16+P+, CD56+P+) in patients with burns injuries in comparation with healthy volunteers, showing the possible prognostic recovery value of these findings. What make this paper unique: our data suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of our patients, indicating the important role of perforin positive cells in naï ; ve immune response and cytotoxic activity of peripheral blood cells of patients with burn injuries.

burns; innate immunity; perforin; NKT cells

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