Citotoksičnost natrij-hipoklorita na kulturama stanica (CROSBI ID 85123)
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Podaci o odgovornosti
Miletić, Ivana ; Knežević, Alena ; Anić, Ivica ; Osmak, Maja ; Šutalo, Jozo ; Blažić-Potočki, Zita
hrvatski
Citotoksičnost natrij-hipoklorita na kulturama stanica
Natrij-hipoklorit (NaOCl) najčešće je rabljena otopina za ispiranje i dezinfekciju tijekom obradbe korijenskoga kanala, unatoč činjenici da može iritirati periapeksno tkivo. Svrha ovoga rada bila je utvrditi citotoksičnost vodene otopine NaOCl na humanim (HeLa) stanicama karcinoma grlića maternice i mišjim (L929) fibroblastima. Ispitane su 0,5%, 1,0%, 2,5% i 5,25% koncentracije NaOCl. Ukupni broj stanica izbrojen je elektroničkim brojačem, a broj živih stanica izbrojen je pod svjetlosnim mikroskopom uz dodatak vitalne nigrosin boje. Najmanje promjene morfologije, broja i postotka živih stanica nađene su kod koncentracije 0,5%, a 5,25%-tna koncentracija uzrokovala je potpuni gubitak HeLa i L929 stanica. Utvrđena je statistički vjerodostojna razlika (p<0,05) između ispitivanih koncentracija NaOCl i kontrolne skupine. Između 1,0% i 2,5% NaOCl razlika u toksičnosti nije bila vjerodostojna za obje stanične linije (p<0.05).
natrij-hipoklorit; kultura stanica; citotoksičnost
nije evidentirano
engleski
The Cytotoxic Effect of Sodium Hypochlorite on Cells Cultures
Sodium hypochlorite (NaOCl) is the most popular irrigation solution used in root canal treatment, in spite of the fact that it may irritate the vital tissues. This study was designed to evaluate the cytotoxic effect of a water solution of sodium hypochlorite on cultures of human cervical carcinoma HeLa cells and mouse L929 fibroblasts by determining the number of vital cells. The tested concentrations of 0.5%, 1.0%, 2.5%, 5.25% were prepared in Eagle's growth medium. The number of cells was counted by electronic counter while the number of viable cells was determined, after the addition of nigrosin dye, by a light microscope. Concentration of 0.5% NaOCl caused the least changes in morphology, number and percentage of viable cells while 5.25% concentration caused total loss of HeLa and L929 cells. All tested concentrations were cytotoxic with significant difference (p<0.05) compared to the control group. However, the results showed that there was no significant difference between 1.0% and 2.5% concentration of NaOCl for both cell lines (p<0.0
sodium hypochlorite; cell culture; cytotoxicity
nije evidentirano
