engleski

Antioxidant capacity in rat brain after ICV treatment with streptozotocin and alloxan – a preliminary study.

Intracerebroventricular ( icv ) administration of betacytotoxic drug streptozotocin ( STZ ) produces long-term and progressive cognitive deficits in rats, as well as deficits in cerebral glucose and energy metabolism. These changes resemble those found in the brain of patients with sporadic Alzheimer’ s disease ( sAD ), therefore STZ-icv treated rats have been proposed as an experimental model of sAD. In this study the antioxidant capacity ( AC ) using manual oxygen radical absorbance capacity ( ORAC ) assay was measured in the rat brain frontoparietal cortex ( FC ) and brainstem-cerebellum part ( BS-CB ) after administration of STZ and another betacytotoxic drug alloxan ( AL ). Region-specific differences of AC were found which were more expressed when hydroxyl radical ( ORAC-OH&ordm ; ) generator was used in the assay. AC against ORAC-OH&ordm ; was significantly lower in BS-CB than in FC of the control rats. ORAC-OH&ordm ; values significantly increased in FC 1 month after icv administration of STZ or combination of glucose transport inhibitor 5-thio-D-glucose +STZ in comparison with the controls. Furthermore, ORAC-OH&ordm ; significantly decreased in BS-CB 3 months following the icv administration of AL but significantly increased following the TG+AL combined treatment in comparison with the controls. However, 3 months following the icv treatment of AL combination with a different glucose transport inhbitor, 3-0-methyl-D-glucose, ORAC-OH&ordm ; values in BS-CB and ORAC-ROO&ordm ; values in FC were significantly decreased in comparison to the controls. Our results sugest that betacytotoxic-icv treatment alters antioxidative defense systems in the brain, which particularly regarding the STZ-icv treatment could be an useful tool in search for the possible new antioxidant treatments of the neurodegenerative disorders such as sAD.

antioxidant capacity; oxygen radical absorbance capacity ( ORAC ) assay; peroxyl radical; hydroxyl radical; oxidative stress; streptozotocin; alloxan; Alzheimer’ s disease

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