AFM as a tool for revealing cell morphology at micro- and nanoscale (CROSBI ID 536437)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Mišić, Tea ; Svetličić, Vesna ; Viljetić, Barbara ; Kalanj Bognar, Svjetlana ; Heffer Lauc, Marija
engleski
AFM as a tool for revealing cell morphology at micro- and nanoscale
Atomic force microscopy (AFM) belongs to the broad family of scanning probe microscopic techniques (SPMs) in which a proximal probe is exploited to investigate the properties of surfaces. AFM provides real topographic images of the sample surfaces in three dimensions with a subangstroem vertical resolution, and with lateral resolution superior to 1 nm. Besides providing topographic data, AFM can also be used for measuring nanomechanical properties of the samples as well as intra- and intermolecular forces. In biological applications, the most appealing advantage of the AFM as a high resolution microscope is that it allows measurements of native biological samples in physiological like condtions, thus obviating complex sample preparation procedures typical for SEM and TEM techniques. In this study, we used AFM in the analysis of human neuroblastoma cell line SH-SY5Y cellular morphology. Neuroblastoma cells were grown on silanized glass coverslips, and cultivated in MEM medium supplemented with 10% fetal bovine serum. For AFM analysis, cells were briefly washed with phosphate buffer saline (PBS) and fixed with 2% paraformaldehyde in PBS. AFM images were collected using Multimode AFM with Nanoscope IIIa controller (Veeco Instruments, Santa Barbara, CA) with a vertical engagement (JV) 125 um scanner. Contact imaging mode was performed in air using minimum force to maintain contact between the probe and the scanned surface. We present AFM images of human neuroblastoma cell line SH-SY5Y. Neuronal-like morphology of SH-SY5Y cells, characterized by cell bodies with neuritic extensions, is viewed in fascinating detail. Cell surface was characterized down to molecular scale (lipid aggregates-membrane rafts). Cell dimensions were accurately measured including cell height, which is useful for determining cell volume. Membrane pores were captured showing excreting activity. Membrane pores were in the size range of 200-500 nm. Studies of different neuronal-like populations fing AFM to be a powerful tool for revealing cellular morphology at micro- and nanoscale.
AFM ; cell morphology
Časopis je indeksiran u Neuroscience Citation indeksu i EMBASE/Excerpta Medica.
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Podaci o prilogu
138-138.
2007.
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objavljeno
Podaci o matičnoj publikaciji
Neurologia Croatica. Supplement
Ivkić, Goran ; Judaš, Miloš ; Klarica, Marijan ; Kostović, Ivica ; Šimić, Goran ; Petanjek, Zdravko
Zagreb: Denona
1331-5196
Podaci o skupu
Croatian Congress of Neuroscience (2 ; 2007)
poster
18.05.2007-19.05.2007
Zagreb, Hrvatska