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Two waves of the nuclear phospholipase C activity in serum-stimulated HL-60 cells during G1 phase of the cell cycle


Lukinović-Škudar, Vesna; Matković, Katarina; Banfić, Hrvoje; Višnjić, Dora
Two waves of the nuclear phospholipase C activity in serum-stimulated HL-60 cells during G1 phase of the cell cycle // Biochimica et Biophysica Acta, 1771 (2007), 4; 514-521 doi:10.1016/j.bbalip.2007.02.002 (međunarodna recenzija, članak, znanstveni)


Naslov
Two waves of the nuclear phospholipase C activity in serum-stimulated HL-60 cells during G1 phase of the cell cycle

Autori
Lukinović-Škudar, Vesna ; Matković, Katarina ; Banfić, Hrvoje ; Višnjić, Dora

Izvornik
Biochimica et Biophysica Acta (1388-1981) 1771 (2007), 4; 514-521

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Phospholipase Cbeta1b; nuclei; serum; cell cycle; G1 phase; HL-60 cells

Sažetak
Phosphatidylinositol-specific phospholipase C (PI-PLC) is activated in cell nuclei during the cell cycle progression. We have previously demonstrated two peaks of an increase in the nuclear PI-PLC activities in nocodazole-synchronized HL-60 cells. In this study, the activity of nuclear PI-PLC was investigated in serum-stimulated HL-60 cells. In serum-starved HL-60 cells, two peaks of the activity of nuclear PI-PLC were detected at 30 minutes and 11 h after the re-addition of serum with no parallel increase in PLC activity in cytosol, postnuclear membranes or total cell lysates. An increase in the serine phosphorylation of b splicing variant of PI-PLCbeta1 was detected with no change in the amount of PI-PLCbeta1b in nuclei isolated at 30 min and 11h after the addition of serum. PI-PLC inhibitor ET-18-OCH3 and MEK inhibitor PD 98059 completely abolished serum-mediated increase at both time-points. The addition of inhibitors either immediately or 6 h after the addition of serum had inhibitory effects on the number of cells entering S phase. These results demonstrate that two waves of nuclear PI-PLCbeta1b activity occur in serum-stimulated cells during G1 phase of the cell cycle and that the later increase in the PLC activity is equally important for the progression into the S phase.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081347-0173 - Funkcija fosfoinozitol 3-kinaze C2 beta u staničnim jezgrama (Hrvoje Banfić, )
108-1081347-1448 - Uloga PLC i Akt u staničnom ciklusu i diferencijaciji leukemija (Dora Višnjić, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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