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Pregled bibliografske jedinice broj: 341007

Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini


Svetec, Ivan-Krešimir; Štafa, Anamarija; Zgaga, Zoran
Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini // Yeast, 24 (2007), 8; 637-652 doi:10.1002/yea.1497 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 341007 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini

Autori
Svetec, Ivan-Krešimir ; Štafa, Anamarija ; Zgaga, Zoran

Izvornik
Yeast (0749-503X) 24 (2007), 8; 637-652

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
gene targeting; gene duplication; aneuploidy; homologous recombination; illegitimate recombination

Sažetak
We investigated the influence of short terminal heterologies on recombination between transforming linear DNA fragments and the yeast Saccharomyces cerevisiae genome. The efficiency of plasmid integration to the CYC1 locus (ends-in assay) was decreased more than five-fold when the size of terminal heterology exceeded 28 nucleotides (nt) and a similar inhibitory effect was also observed in the ends-out assay (replacement of the ura3-52 allele by the UPIA3 gene). Plasmid integration occurred almost exclusively in the target homology and was accompanied by excessive degradation of the heterollogous termini. Illegitimate integrations were much more frequent in the ends-out transformation in both the absence (8.9%) and the presence (23.7%) of 45/46 heterologous nucleotides at the ends of the transforming fragment. Interestingly, only about 60% of transformants arose by simple gene replacement, regardless of the presence of heterologous ends, whereas more complex interactions resulted in gene or whole chromosome duplications. Our results warn that different genetic alterations may be introduced in the host strain during ends-out transformation but also indicate possible mechanisms for formation of duplications in the genome.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
058-0580477-2258 - Palindromi u genomima i mehanizmi zamjene gena u kvasca (Krešimir Svetec, Ivan, MZOS ) ( POIROT)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Anamarija Štafa (autor)

Avatar Url Ivan Krešimir Svetec (autor)

Avatar Url Zoran Zgaga (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Svetec, Ivan-Krešimir; Štafa, Anamarija; Zgaga, Zoran
Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini // Yeast, 24 (2007), 8; 637-652 doi:10.1002/yea.1497 (međunarodna recenzija, članak, znanstveni)
Svetec, I., Štafa, A. & Zgaga, Z. (2007) Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini. Yeast, 24 (8), 637-652 doi:10.1002/yea.1497.
@article{article, author = {Svetec, Ivan-Kre\v{s}imir and \v{S}tafa, Anamarija and Zgaga, Zoran}, year = {2007}, pages = {637-652}, DOI = {10.1002/yea.1497}, keywords = {gene targeting, gene duplication, aneuploidy, homologous recombination, illegitimate recombination}, journal = {Yeast}, doi = {10.1002/yea.1497}, volume = {24}, number = {8}, issn = {0749-503X}, title = {Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini}, keyword = {gene targeting, gene duplication, aneuploidy, homologous recombination, illegitimate recombination} }
@article{article, author = {Svetec, Ivan-Kre\v{s}imir and \v{S}tafa, Anamarija and Zgaga, Zoran}, year = {2007}, pages = {637-652}, DOI = {10.1002/yea.1497}, keywords = {gene targeting, gene duplication, aneuploidy, homologous recombination, illegitimate recombination}, journal = {Yeast}, doi = {10.1002/yea.1497}, volume = {24}, number = {8}, issn = {0749-503X}, title = {Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini}, keyword = {gene targeting, gene duplication, aneuploidy, homologous recombination, illegitimate recombination} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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