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TGF-ß Expression in patients with focal segmental glomerulosclerosis with regard to histologic changes and ACE gene polymorphism

TGF-ß is a multifactorial cytokine with an important role in formation of extracellular matrix whose over-expression results in glomerulosclerosis and tubulointerstitial fibrosis accelerating renal disease progression. ACE gene polymorphism can be assumed to affect it since angiotensin II stimulates its expression. The aim of the study was to analyze TGF-ß expression with regard to the rate of histologic changes and ACE gene polymorphism. The TGF-ß expression determined using murine monoclonal antibodies was estimated semiquantatively (no expression-N, weak-W, moderate/strong-M) in 21 patients with FSGS. Histologic changes were classified as mild, moderate and severe. ACE gene polymorphism was determined by PCR method. TGF-ß expression was not found in 4 out of 5 patients with mild lesion, but it was found in 5 out of 9 patients with moderate lesion. M was present in 3 out of 7 patients with severe lesion, and W was present in 4 patients. DD genotype was found in 6 patients, with the following TGF-ß expression:N-1, W-3, M-2, while the distribution in 11 patients with ID genotype was N-1, W-3, and M-4. N-1, W-2 and M-1 were established in 4 patients with II genotype. Increased TGF-ß expression was observed in patients with FSGS and severe histologic changes. ACE gene polymorphism does not show clear correlation with TGF-ß expression, which is possibly a consequence of a low number of patients.

TGFß expression; focal segmental glomerulosclerosis; ACE gene polymorphism

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