hrvatski

Total glutathione S-transferase (GSTs) activity in serum of healthy subjects and patients with stable COPD

Cronic obstructive pulmonary disease (COPD)is an obstructive airway disorder characterizied by a slowly progressive and irreversible decrease in FEV1. More than 90% of patients with COPD are smokers, but not all smokers develop COPD. Cigarette smoke containing more than 1016-1017 oxidant molecules per puff and is an important cause of oxidative stress in COPD. Glutathione S-transferases (GSTs) protect cell against numerous oxidant generating compounds from cigarette smoke. The aim of the study was to assess the possible changes in catalitic concretations of GSTs, alpha 1-antitrypsin (AAT) and lactate dehydrogenase (LDH) in serum of elderly men with stable COPD (smokers and ex-smokers) in comparison to age mached healthy control men (control group, mean age 63± ; ; 5: 19 non-smokers, 16 ex-smokers and 25 current life-long smokers. Group 2 included 30 patients with stable COPD (mean age 65± ; ; 7 years): 12 ex-smokers, 14 current life-long smokers and 4 that had never smoked. Blood was collected by venipuncture during morning hours and prior to administration of any medication. GSTs activity was determined by a new, rapid spectrophotometric method described by Habdous et al. AAT concentration was determined by RID technique. LDH was measured by standard method using available tests for Olympus AU 400 analyzer. Analysis of GSTs activity in serum of all COPD patient subgroups showed lower values (median: 67 U/L (21 – 104 U/L) as compared with respective control group (median: 68.5 U/L (38 – 111 U/L) but the differences were not statistically significant. This decreasing trend of all GSTs activity in COPD patients could probably be explained by the fall of GSH level in COPD, since GSH is a cofactor for various enzymes that decrease oxidative stress. The rwsults on total AAT (acute phase protein) concentrations in COPD patients where significantly higher as compared with control group (p<0.05). LDH activity was measured as a marker of enhanced anaerobic metabolism. The value of total LDH activity in COPD patients was statistically higher as compared with controls total (p<0.05). From ROC analysis we concleded that GSTs, AAT and LDH had low accuracy in differentiating patients with stable COPD and healthy controls.

COPD; inflammation; oxidative stresss; glutathione S-transferases (GST); alpha-1-antitrypsin (AAT); lactate dehydogenase (LDH).

nije evidentirano

njemački

Total glutathione S-transferase (GSTs) activity in serum of healthy subjects and patients with stable COPD

Cronic obstructive pulmonary disease (COPD)is an obstructive airway disorder characterizied by a slowly progressive and irreversible decrease in FEV1. More than 90% of patients with COPD are smokers, but not all smokers develop COPD. Cigarette smoke containing more than 1016-1017 oxidant molecules per puff and is an important cause of oxidative stress in COPD. Glutathione S-transferases (GSTs) protect cell against numerous oxidant generating compounds from cigarette smoke. The aim of the study was to assess the possible changes in catalitic concretations of GSTs, alpha 1-antitrypsin (AAT) and lactate dehydrogenase (LDH) in serum of elderly men with stable COPD (smokers and ex-smokers) in comparison to age mached healthy control men (control group, mean age 63± ; ; 5: 19 non-smokers, 16 ex-smokers and 25 current life-long smokers. Group 2 included 30 patients with stable COPD (mean age 65± ; ; 7 years): 12 ex-smokers, 14 current life-long smokers and 4 that had never smoked. Blood was collected by venipuncture during morning hours and prior to administration of any medication. GSTs activity was determined by a new, rapid spectrophotometric method described by Habdous et al. AAT concentration was determined by RID technique. LDH was measured by standard method using available tests for Olympus AU 400 analyzer. Analysis of GSTs activity in serum of all COPD patient subgroups showed lower values (median: 67 U/L (21 – 104 U/L) as compared with respective control group (median: 68.5 U/L (38 – 111 U/L) but the differences were not statistically significant. This decreasing trend of all GSTs activity in COPD patients could probably be explained by the fall of GSH level in COPD, since GSH is a cofactor for various enzymes that decrease oxidative stress. The rwsults on total AAT (acute phase protein) concentrations in COPD patients where significantly higher as compared with control group (p<0.05). LDH activity was measured as a marker of enhanced anaerobic metabolism. The value of total LDH activity in COPD patients was statistically higher as compared with controls total (p<0.05). From ROC analysis we concleded that GSTs, AAT and LDH had low accuracy in differentiating patients with stable COPD and healthy controls.

COPD; inflammation; oxidative stresss; glutathione S-transferases (GST); alpha-1-antitrypsin (AAT); lactate dehydogenase (LDH).

nije evidentirano