engleski

Differential leukocyte count in haematology patients - automatic analyser of blood cells versus analysis of light microscope

Aim of the study. To analyse the possibility of recognizing pathological blood cells in patients with haematological neoplasm. Material and Methods. The analysis included 52 samples of peripheral blood in patients with haematological neoplasm. A light microscope analysis was conducted using peripheral blood smears stained using the Papenheim method, and, veinal blood with heparin for an analysis using a haematology analyser. Samples in which the counter noted the presence of abnormal distribution of blood cells were analysed. Results. The light microscope indicated the existence of blasts in 28 blood smear samples, whereas the counter identified 13 as containing blasts ; in 15 cases the counter specified them as atypical lymphocytes. In 5 cases there were no comments on findings, however, this accounted for only 1 % of blasts in blood smears. There was indication of immature granulocytes in 31 samples and in 19 cases they were noted in the blood smear ; whereas there was no shift in the granulocyte order in 13 cases, a shift was noted in 8 blood smear, which was not commented by the analyser. Atypical lymphatic cells in both cases were found in 9 samples ; in 15 samples the above mentioned blasts were discovered, and in 10 samples there was no comment. In cases where there was a warning indicating the presence of Iymphoblasts, the samples contained plasma cells, lymphoplasmacytoid forms and other atypical lymphatic cells. A counter warning did not herald the presence of erythroblasts in the smear. Conclusion. The haematology counter is an invaluable and practical tool for quantifying particular cell types due to its quantitative parameters for blood analyses, and it can analyse a vast number of cells within a short period of time. However, although it cannot completely replace the light microscope in analysing blood samples from patients with haematological illnesses where there is a presence of various pathological white blood cells during illness, treatment and rehabilitation, it is an aid that provides comments on the presence of atypical forms and helps to separate samples for further analysis.

Pathological blood cells; light microscope analysis; haematology analyser

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