engleski

Muscarinic activation of DAG signalling in the ovary: induction by hemicastration

It seems that hemicastration induces functional chages of ovarian muscarinic receptors *Can J Physiol Pharmacol 1994; 72(SUppl 1):379*. In the present study, 1,2-diacylglycerol (DAG) levels in the remnant ovaries were, as measured 15 minutes following the operation, increased 8by 2-3 fold), especially (by 3-5 fold) if organs were incubated with carbachol (50 uM). The hemicastration-induce increase was inhibited by atropine (iv) or botulinum toxin (into ovarian bursa) pretreatments, or, by abdominal vagotomy. The effect of carbachol was inhibited by atropine, or by abdominal vagotomy performed prior to hemicastration, but was preserved if bagotomy was performed 10 minutes after hemicastration. Carbachol, atropine or botulinum toxin had no effects on DAG levels in the control organs (sham-operated rats). The results suggest that hemicastration induces a) DAG accumulation in the remnant ovary via innervated muscarinic receptors; b) coupling or the receptors to DAG signalling for which a vagus-dependent information is needed.

ovary; hemicastration; phosphoinositide signalling

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