Development and prevalidation of a method for phenol determination by solid-phase spectrophotometry (CROSBI ID 138840)
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Podaci o odgovornosti
Vuković, Jadranka ; Matsuoka, Shiro ; Yoshimura, Kazuhisa ; Grdinić, Vladimir ; Jurišić Grubešić, Renata
engleski
Development and prevalidation of a method for phenol determination by solid-phase spectrophotometry
A simple, sensitive and rapid solid-phase spectrophotometric procedure was developed for the determination of traces of phenol with 4-aminoantipyrine as a reagent (AAP-SPS), and the optimal experimental conditions were established. This method was performed by sorption and direct absorbance measurements of the product phenol-AAP sorbed on the anion-exchanger Dowex 1-X4 (0.2 g) at 495 nm (absorption maximum) and 700 nm (non-absorption wavelength). The sensitivity offered by the AAP-SPS procedure was higher by a factor of 40 compared with the respective conventional spectrophotometric method. Metrological characteristics were established using a prevalidation strategy. The AAP-SPS procedure is characterized by a linear calibration function in the working range of 0.05-0.50 mu mol, low standard deviation of procedure (+/- 0.012), low limit of determination (0.021 mu mol), and favorable random (+/- 0.85 to +/- 11.27%) and systematic deviations (-4.55 to +/- 11.50%). Moreover, the accuracy of the system investigated by the recovery test is acceptable (99-102%). Favorable working and performance characteristics make the new SPS method ideal for phenol monitoring in pharmaceutical preparations as well as other matrices.
phenol determination; prevalidation strategy; solid-phase spectrophotometry; 4-aminoantipyrine; pharmaceutical application
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