engleski

Perforin expression in peripheral blood lymphatic cells of patients subjected to laparoscopic or open cholecystectomy

Perforin (P)-related characteristics of cytotoxic T lymphocytes and natural killer cells were investigated in peripheral blood of patients subjected to open (OC ; n=23) or laparoscopic cholecystectomy (LC ; n=21) and healthy controls (n=20). Blood samples were obtained preoperatively and 24 h after the surgeries and the data were correlated with the intensity of cholestasis and concomitant inflammation, determined by functional hepatic tests. Postoperative differences were found to be minimal: OC decreased only the percentage of CD56+ cells, while LC decreased the fraction of CD8+P+ cells and augmented the mean fluorescence intensity of P in CD56 cells. Patients elected for OC had, however, higher preoperative numbers of total P+, CD3+P+ and CD4+P+ cells than patients elected for LC and healthy controls, while both groups of patients, preoperatively, had lower fraction of CD16+P+ and CD56+P+ cells. These changes were in high correlation with blood concentrations of CRP, AP and ALT, emphasizing the link between the preoperative cholestasis and inflammation and P-dependent cytotoxic mechanisms.

klasična i laparoskopska kolecistectomija; perforin; citotoksični limfociti; kolestaza.

Article ID 125152.