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Pregled bibliografske jedinice broj: 332466

Cytotoxicity of Selected Pyridinium Oximes in Human SH-SY5Y Neuroblastoma Cell Line


Kalanj Bognar, Svjetlana; Foretić, Blaženka; Vukelić, Željka; Gulin, Tonko; Ježek, Davor
Cytotoxicity of Selected Pyridinium Oximes in Human SH-SY5Y Neuroblastoma Cell Line // Croatica Chemica Acta, 81 (2008), 1; 67-71 (međunarodna recenzija, članak, znanstveni)


Naslov
Cytotoxicity of Selected Pyridinium Oximes in Human SH-SY5Y Neuroblastoma Cell Line

Autori
Kalanj Bognar, Svjetlana ; Foretić, Blaženka ; Vukelić, Željka ; Gulin, Tonko ; Ježek, Davor

Izvornik
Croatica Chemica Acta (0011-1643) 81 (2008), 1; 67-71

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aldoximes; in vitro cytotoxicity; morphological analysis

Sažetak
Pyridinium oximes are pharmacologically important nucleophylic agents acting as effective antidotes against poisoning by organophosphorus compounds that inhibit acetylcholinesterase (AChE ; EC 3.1.1.7.). In this study, the cytotoxicity of 1-phenacylpyridinium-4-aldoxime chloride (FEPA-4) was analyzed in human SH-SY5Y neuroblastoma cell line highly expressing acetylcholinesterase. The concentrations of 0.5, 1, 2 and 4 mmol dm– 3 and time-dependent effects at 1, 3, 6, 12 and 24 hours were tested in comparison with non-treated cells. In addition, neuroblastoma cells were treated with a well known antidote 1, 1'-bis(pyridinium-4-aldoxime)trimethylene dibromide (TMB-4) at the concentration of 0.8 mmol dm– 3, the highest studied concentration which in several non-neural cellular models induced no cytotoxicity. Cytotoxic effects i.e. altered cellular morphology and decreased cellular volume density quantified by stereological method were observed in FEPA-4 treated cells, while no cytotoxic effect was observed for 0.8 mmol dm– 3 TMB-4. Possible mechanisms of observed FEPA-4 cytotoxicity in neuroblastoma cells are discussed.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
108-1080399-0383 - Muški i ženski spolni sustav: razvoj, normalna histofiziologija i neplodnost (Davor Ježek, )
108-1081870-1877 - Uloga membranskih lipida u moždanom razvitku, starenju i neurodegeneraciji (Svjetlana Kalanj-Bognar, )
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Željka Vukelić, )
108-1193079-3070 - Kompleksi željeza i biološki aktivnih liganada (Blaženka Foretić, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • CA Search (Chemical Abstracts)
  • Science Citation Index