engleski

Induction of organogenesis and influence of selective treatments and antimicrobial substances on adventitious roots in Coleus blumei Benth.

Organogenesis of Coleus blumei leaf, nodal and internodal explants was analyzed. Leaf explants might be the best for incorporation into a transformation system where transgenic roots are expected. Antibacterial substances cefotaxime, vancomycine and plant preservative mixture (PPM) did not influence root development and are good for bacteria elimination after Coleus blumei transformation. Transgenic roots could be regenerated on media with 20 mg/l kanamycin, 70 mg/l neomycin, 2 mg/l geneticin, 10 mg/l hygromycin or 1 mg/l glufosinate ammonium.

organogenesis; selective treatments; adventitious roots; Coleus blumei

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