Napredna pretraga

Pregled bibliografske jedinice broj: 330505

A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate


Kraljević Pavelić, Sandra; Čačev, Tamara; Kralj, Marijeta
A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate // Cancer Gene Therapy, 15 (2008), 9; 576-590 doi:10.1038/cgt.2008.28 (međunarodna recenzija, članak, znanstveni)


Naslov
A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate

Autori
Kraljević Pavelić, Sandra ; Čačev, Tamara ; Kralj, Marijeta

Izvornik
Cancer Gene Therapy (0929-1903) 15 (2008), 9; 576-590

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Apoptosis; GADD45a; p21waf1/cip1; HEp-2 cells; cisplatin; methotrexate

Sažetak
We investigated the effects of p21waf1/cip1 gene over-expression in human laryngeal squamous carcinoma cells HEp-2 lacking p53 protein expression on apoptosis induction upon the treatment with two commonly used chemotherapeutic agents, cisplatin and methotrexate. For that purpose, we employed cDNA-arrays and qPCR to monitor gene expression upon treatment with AdCMV-p21 alone or in combination with the chemotherapeutic compounds. We found that p21waf1/cip1 gene over-expression provoked apoptosis of HEp-2 through the induction of the TNFRSF9 gene and activation of caspase 7. Additionally, we have proved that p21waf1/cip1 can assume a dual role in apoptosis in the same cell system depending on the chemotherapeutic agent: its over-expression enhances apoptosis in cisplatin-treated cells and attenuates apoptotic signals in methotrexate-treated cells. The observed dual role of p21waf1/cip1 was in direct correlation with the modulation of caspase 3 and 7 activation and changes in the expression of GADD45a gene. The results presented herein encourage future use of targeted p21waf1/cip1 gene therapy in cancer treatment in a well-defined therapeutic and genetic context.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2393 - Molekularna obilježja miofibroblasta Dupuytrenove bolesti (Krešimir Pavelić, )
098-0982464-2508 - Molekularna genetika i farmakogenetika gastrointestinalnih tumora (Sanja Kapitanović, )
098-0982464-2514 - Uloga različitih mehanizama odgovora stanica na terapiju oštećenjem DNA (Marijeta Kralj, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati