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Pregled bibliografske jedinice broj: 330505

A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate


Kraljević Pavelić, Sandra; Čačev, Tamara; Kralj, Marijeta
A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate // Cancer gene therapy, 15 (2008), 9; 576-590 doi:10.1038/cgt.2008.28 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 330505 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate

Autori
Kraljević Pavelić, Sandra ; Čačev, Tamara ; Kralj, Marijeta

Izvornik
Cancer gene therapy (0929-1903) 15 (2008), 9; 576-590

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Apoptosis ; GADD45a ; p21waf1/cip1 ; HEp-2 cells ; cisplatin ; methotrexate

Sažetak
We investigated the effects of p21waf1/cip1 gene over-expression in human laryngeal squamous carcinoma cells HEp-2 lacking p53 protein expression on apoptosis induction upon the treatment with two commonly used chemotherapeutic agents, cisplatin and methotrexate. For that purpose, we employed cDNA-arrays and qPCR to monitor gene expression upon treatment with AdCMV-p21 alone or in combination with the chemotherapeutic compounds. We found that p21waf1/cip1 gene over-expression provoked apoptosis of HEp-2 through the induction of the TNFRSF9 gene and activation of caspase 7. Additionally, we have proved that p21waf1/cip1 can assume a dual role in apoptosis in the same cell system depending on the chemotherapeutic agent: its over-expression enhances apoptosis in cisplatin-treated cells and attenuates apoptotic signals in methotrexate-treated cells. The observed dual role of p21waf1/cip1 was in direct correlation with the modulation of caspase 3 and 7 activation and changes in the expression of GADD45a gene. The results presented herein encourage future use of targeted p21waf1/cip1 gene therapy in cancer treatment in a well-defined therapeutic and genetic context.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
098-0982464-2508 - Molekularna genetika i farmakogenetika gastrointestinalnih tumora (Kapitanović, Sanja, MZOS ) ( POIROT)
098-0982464-2514 - Uloga različitih mehanizama odgovora stanica na terapiju oštećenjem DNA (Kralj, Marijeta, MZOS ) ( POIROT)
098-0982464-2393 - Molekularna obilježja miofibroblasta Dupuytrenove bolesti

Ustanove:
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Kraljević Pavelić, Sandra; Čačev, Tamara; Kralj, Marijeta
A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate // Cancer gene therapy, 15 (2008), 9; 576-590 doi:10.1038/cgt.2008.28 (međunarodna recenzija, članak, znanstveni)
Kraljević Pavelić, S., Čačev, T. & Kralj, M. (2008) A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate. Cancer gene therapy, 15 (9), 576-590 doi:10.1038/cgt.2008.28.
@article{article, year = {2008}, pages = {576-590}, DOI = {10.1038/cgt.2008.28}, keywords = {Apoptosis, GADD45a, p21waf1/cip1, HEp-2 cells, cisplatin, methotrexate}, journal = {Cancer gene therapy}, doi = {10.1038/cgt.2008.28}, volume = {15}, number = {9}, issn = {0929-1903}, title = {A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate}, keyword = {Apoptosis, GADD45a, p21waf1/cip1, HEp-2 cells, cisplatin, methotrexate} }
@article{article, year = {2008}, pages = {576-590}, DOI = {10.1038/cgt.2008.28}, keywords = {Apoptosis, GADD45a, p21waf1/cip1, HEp-2 cells, cisplatin, methotrexate}, journal = {Cancer gene therapy}, doi = {10.1038/cgt.2008.28}, volume = {15}, number = {9}, issn = {0929-1903}, title = {A dual role of p21waf1/cip1 gene in apoptosis of HEp-2 treated with cisplatin or methotrexate}, keyword = {Apoptosis, GADD45a, p21waf1/cip1, HEp-2 cells, cisplatin, methotrexate} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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