engleski

Tonsillar macrophages and dendritic cells in recurrent tonsillitis

Characteristics of tonsillar macrophages (Mψ s) in children, after recurrent tonsillitis were studied in vitro. Dendritic cells (DCs) were isolated from classical tissue Mψ s by two step dissociation of tonsillar tissue. DCs and lymphocytes were released from tonsillar tissue by mechanical dissociation, while Mψ s were obtained by the collagenase digestion of tissue fragments left after the mechanical dissociation. Pure Mψ s and DCs enriched up to 80% were prepared by density gradient centrifugation and adherence to glass. The adherence, spreading, and ingestion properties of Mψ s, were examined and compared to corresponding characteristics of DCs and monocytes derived from the same child. Enhanced spreading and ingestion of isolated Mψ s were interpreted as signs of their in vivo activation by various antigens and activating stimuli released during inflammation.

Tonsillar macrophages; dendritic cells; tonsillitis

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