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Pregled bibliografske jedinice broj: 329436

Tenocyclidine treatment in soman poisoned rats - intriguing results on genotoxicity versus protection


Petek, Maja Jelena; Berend, Suzana; Kopjar, Nevenka; Želježić, Davor; Mladinić, Marin; Radić, Božica; Lucić Vrdoljak, Ana
Tenocyclidine treatment in soman poisoned rats - intriguing results on genotoxicity versus protection // Acta Biochimica Polonica, 55 (2008), 1; 97-105 (međunarodna recenzija, članak, znanstveni)


Naslov
Tenocyclidine treatment in soman poisoned rats - intriguing results on genotoxicity versus protection

Autori
Petek, Maja Jelena ; Berend, Suzana ; Kopjar, Nevenka ; Želježić, Davor ; Mladinić, Marin ; Radić, Božica ; Lucić Vrdoljak, Ana

Izvornik
Acta Biochimica Polonica (0001-527X) 55 (2008), 1; 97-105

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Tenocyclidine; soman; cholinesterase activity; rat; plasma; brain; genotoxicity; comet assay

Sažetak
This study was aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with &#188; and &#189; of LD50 of soman. Possible genotoxic effects of TCP in white blood cells and brain tissue were also studied. Results were compared with previous findings on the adamantyl tenocyclidine derivative TAMORF. TCP given alone as pretreatment, 5 min before soman, seems to be superior in the protection of cholinesterase (ChE) catalytic activity in the plasma than in brain, especially after administration of the lower dose. Plasma activities of enzyme significantly increased as compared to corresponding controls 30 min (P < 0.001) and 24h (P = 0.0043). TCP and atropine, given as therapy, were more effective than TCP administered solely as a pretreatment. Activities of enzyme significantly increased as compared to controls 30 min (P = 0.046) and 24h (P < 0.001) when &#188; LD50 dose of soman was administered. Using the alkaline comet assay acceptable genotoxicity of TCP was observed. However, controversial role of TCP in brain protection of soman-poisoned rats should be further studied.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
022-0222148-2139 - Terapijski učinak novosintetiziranih spojeva pri otrovanju organofosfatima (Ana Lucić Vrdoljak, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE