engleski

Tenocyclidine treatment in soman poisoned rats - intriguing results on genotoxicity versus protection

This study was aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with &#188; and &#189; of LD50 of soman. Possible genotoxic effects of TCP in white blood cells and brain tissue were also studied. Results were compared with previous findings on the adamantyl tenocyclidine derivative TAMORF. TCP given alone as pretreatment, 5 min before soman, seems to be superior in the protection of cholinesterase (ChE) catalytic activity in the plasma than in brain, especially after administration of the lower dose. Plasma activities of enzyme significantly increased as compared to corresponding controls 30 min (P < 0.001) and 24h (P = 0.0043). TCP and atropine, given as therapy, were more effective than TCP administered solely as a pretreatment. Activities of enzyme significantly increased as compared to controls 30 min (P = 0.046) and 24h (P < 0.001) when &#188; LD50 dose of soman was administered. Using the alkaline comet assay acceptable genotoxicity of TCP was observed. However, controversial role of TCP in brain protection of soman-poisoned rats should be further studied.

tenocyclidine; soman; cholinesterase activity; rat; plasma; brain; genotoxicity; comet assay

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