engleski

The changes of serum enzyme activity as an indicator of injuries in irradiated chickens

Introduction: Many authors have reported that organic lesions and metabolic disorders of many organs, especially the liver, are followed by changes of some enzyme activities in blood plasma of domestic animals and poultry. It is also known that the liver in birds is equally affected by radiation as the intestine, bone-marrow and sex glands, which is different in mammals. In our previous investigation we showed that the activities of some enzymes in blood plasma of chickens are significantly changed after a single parenteral injection of 32P. In this paper an attempt was made to investigate the influence of gamma ray irradiation of whole body of chickens upon activity of several enzyme (enzymatic profile) in blood plasma. We also wanted to evaluate whether investigation of the enzymatic profile in blood plasma can help in the diagnosis of organic or functional liver damages caused by gamma ray in the chickens.Materials and Methods: The experiments were performed on hybrid chickens of heavy Jata breeds of booth sexes. Chickens were irradiated by gamma ray in the dose of 7, 23± 0.95 Gy. Blood samples were taken from the wing vein on days 1, 3, 5, 7, 9 and 15 after irradiation.The activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT), leucine aminopeptidase (LAP), lactate dehydrogenase (LDH) and alpha-hydroxy-butyrate dehydrogenase (HBDH) in blood plasma of irradiated chicken was determined spectrophotometrically by using Boehringer Mannheim GmbH optimized kits. At the end of the experiment all birds were sacrificed and the organs were pathomorphologically and histologically investigated. Results: Throughout the experimental period, only ALT and GGT activity did not statistically significantly change. All other enzymes activity showed a decreased tendency during the experiment ; statistically significant decreases were recorded as follows: on the 1st day of the experiment – LAP and LDH activity ; on the 3rd day - LAP activity ; on the 5th day - LDH activity ; on the 7th day – AST, LDH and HBDH activity, on the 9th day – LAP and HBDH activity, and on the 15th day – LAP activity.Conclusion: The determination of a so-called enzyme profile in blood plasma which include measurement AST, LAP, LDH and HBDH activities may serve as an additional test for functional liver damages in chickens caused by ionizing radiation before the appearance of clinical simptoms.

enzyme; blood plasma; ionizing radiation; chicken

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