engleski

Effect of thiol compound during in vitro culture on bovine embryo development

The purpose of this study was to evaluate weather the addition of cysteamine during in vitro culture (IVC) of bovine embryos enhances embryo development. Cumulus-oocytes complexes collected from ovaries of slaughtered donors were matured in vitro in TCM 199 supplemented with 10% FCS, FSH/LH (Pergonal, 75/75 IU, Serono), 1 µ ; g/ml estradiol-17ß and 100 µ ; M cysteamine for 24 hours. After IVM the oocytes were fertilized (Day 0). Following fertilization embryos were cultured in SOFaaBSA without glucose for 48 h. Day 3 embryos were transfered to SOFaaBSA with 1, 5 mM glucose (SOF g) supplemented with 0 or 100 µ ; M cysteamine and cultured for 48 h. After this, embryos were transferred in SOF g without cysteamine and cultured until Day 9. Total of 474 oocytes were used in five repetitions. The endpoints for evaluation were: Day 2 cleavage rate ; Day 7 blastocyst rate and Day 9 hatching rate. The percentage of embryos that developed to the blastocyst stage on Day 7 was significantly higher (p<0, 05) in cysteamine supplemented group (9, 25% vs. 20, 27%). Moreover, the addition of cysteamine during IVC significantly increased the proportion of transferable quality (Grade 1 and 2) embryos (p<0, 05). However, no differences were observed in hatching rate between unsupplemented and supplemented group (5, 70% vs. 10, 58%). The present study demonstrated that the addition of thiol compound such as cysteamine to the IVC medium improves bovine in vitro embryo production.

bovine; IVC; cysteamine; embryo development

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