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Pregled bibliografske jedinice broj: 327958

Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function


Romac, Pavle; Žanić-Grubišić, Tihana; Ježek, Davor; Vučić, Marijana; Čulić, Ognjen
Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function // Croatica Chemica Acta, 81 (2008), 1; 169-176 (međunarodna recenzija, članak, znanstveni)


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Naslov
Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function

Autori
Romac, Pavle ; Žanić-Grubišić, Tihana ; Ježek, Davor ; Vučić, Marijana ; Čulić, Ognjen

Izvornik
Croatica Chemica Acta (0011-1643) 81 (2008), 1; 169-176

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Spermatozoa; Ecto-NTPDase; 5'-nucleotidase; Capacitation

Sažetak
Evidence is presented for the existence of ectonucleotidases on the membrane of intact human spermatozoa. Enzymes hydrolyze extracellular ATP, ADP and AMP and hence could be described as ecto-NTPDase and ecto-5'-nucleotidase. Suramin, Cibacron 3GA and DIDS, well known ectoNTPDase inhibitors, caused inhibition of the observed enzyme activity. Enzymatic hydrolysis of ATP, ADP and AMP follows simple Michaelis-Menten kinetics with similar enzyme affinity for all three substrates (Km for ATP, ADP and AMP were 0.395&plusmn ; ; ; ; ; 0.027, 0.401&plusmn ; ; ; ; ; 0.031, 0.517&plusmn ; ; ; ; ; 0.038 mmole dm-3, respectively). Influence of extracellular ATP, AMP, adenosine and cAMP on the parameters of sperm velocity and acrosome reaction was also examined. In normozoospermic samples, ATP and cAMP induced an increase in the amplitude of lateral head displacement and percentage of acrosomally reacted cells, but not in sperm velocity. However, adenosine and AMP enhanced sperm velocity, without influencing the acrosome reaction. These results show that ATP and adenosine regulate sperm motility parameters in different ways.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
006-0061245-0977 - Molekularni mehanizmi patogeneze kronične opstrukcijske bolesti pluća (Žanić-Grubišić, Tihana, MZOS ) ( POIROT)
045-1191348-0139 - Biomolekule guanidinskog tipa u šećernoj bolesti (Vučić Lovrenčić, Marijana, MZOS ) ( POIROT)
108-1080399-0383 - Muški i ženski spolni sustav: razvoj, normalna histofiziologija i neplodnost (Ježek, Davor, MZOS ) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb


Citiraj ovu publikaciju:

Romac, Pavle; Žanić-Grubišić, Tihana; Ježek, Davor; Vučić, Marijana; Čulić, Ognjen
Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function // Croatica Chemica Acta, 81 (2008), 1; 169-176 (međunarodna recenzija, članak, znanstveni)
Romac, P., Žanić-Grubišić, T., Ježek, D., Vučić, M. & Čulić, O. (2008) Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function. Croatica Chemica Acta, 81 (1), 169-176.
@article{article, year = {2008}, pages = {169-176}, keywords = {Spermatozoa, Ecto-NTPDase, 5'-nucleotidase, Capacitation}, journal = {Croatica Chemica Acta}, volume = {81}, number = {1}, issn = {0011-1643}, title = {Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function}, keyword = {Spermatozoa, Ecto-NTPDase, 5'-nucleotidase, Capacitation} }
@article{article, year = {2008}, pages = {169-176}, keywords = {Spermatozoa, Ecto-NTPDase, 5'-nucleotidase, Capacitation}, journal = {Croatica Chemica Acta}, volume = {81}, number = {1}, issn = {0011-1643}, title = {Hydrolysis of extracellular adenine nucleotides by human spermatozoa: regulatory role of ectonucleotidases in sperm function}, keyword = {Spermatozoa, Ecto-NTPDase, 5'-nucleotidase, Capacitation} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





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