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Cytotoxicity of composite materials polymerized with LED curing units


Knežević, Alena; Želježić, Davor; Kopjar, Nevenka; Tarle, Zrinka
Cytotoxicity of composite materials polymerized with LED curing units // Operative Dentistry, 33 (2008), 1; 23-30 doi:10.2341/07-16 (međunarodna recenzija, članak, znanstveni)


Naslov
Cytotoxicity of composite materials polymerized with LED curing units

Autori
Knežević, Alena ; Želježić, Davor ; Kopjar, Nevenka ; Tarle, Zrinka

Izvornik
Operative Dentistry (0361-7734) 33 (2008), 1; 23-30

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cytotoxicity; composite materials; curing light

Sažetak
The proper intensity and illumination time of a curing light is of great importance for the complete polymerization of resin composites and long-lasting resin composite restorations. Inadequately cured resin composites can have a cytotoxic effect on pulp tissue by releasing unreacted monomers. This study determined whether there is any difference in cytotoxicity between composite materials illuminated with different curing modes of LED curing units. Thin layers of two composite materials were polymerized using three different modes of the Bluephase C8 LED curing unit: a high intensity mode (HIP-800 mW/cm2, 20 seconds), a soft-start mode (SOF-650 mW/cm2 first 5 seconds, 800 mW/cm2 next 25 seconds) and a low intensity mode (LOP-650 mW/cm2, 30 seconds). Lymphocyte cultures were treated with both polymerized and unpolymerized composites using one of the modes stated above. Cells were analyzed using the trypan blue exclusion test, the acridine orange/ethidium bromide dying technique and an alkaline comet assay. Significant cytotoxicity was observed for 120 mg of unpolymerized composites and those polymerized with the HIP polymerization mode. A significant level of DNA damage was detected for 120 mg of unpolymerized composites. However, curing via the LOP program exhibited the lowest genotoxicity. Longer curing time with lower intensity results in less cytotoxicity than shorter curing exposure using a higher intensity of light emitted from the curing light source.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Dentalna medicina



POVEZANOST RADA


Projekt / tema
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
065-0352851-0410 - Nanostruktura restaurativnih materijala i interakcije s tvrdim zubnim tkivima (Zrinka Tarle, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Stomatološki fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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