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Pregled bibliografske jedinice broj: 327560

Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment


Šegvić Klarić, Maja; Rumora, Lada; Ljubanović, Danica; Pepeljnjak, Stjepan
Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment // Archives of Toxicology, 82 (2008), 4; 247-255 (međunarodna recenzija, članak, znanstveni)


Naslov
Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment

Autori
Šegvić Klarić, Maja ; Rumora, Lada ; Ljubanović, Danica ; Pepeljnjak, Stjepan

Izvornik
Archives of Toxicology (0171-9750) 82 (2008), 4; 247-255

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Fumonisin B1; Beauvericin; Ochratoxin A; Necrosis; Apoptosis; Caspase-3; Mycotoxin interactions

Sažetak
The objective of this study was to determine individual and combined effects of fumonisin B(1) (FB(1)), beauvericin (BEA) and ochratoxin A (OTA) on porcine kidney epithelial PK15 cell survival by measuring lactate dehydrogenase (LDH) activity, apoptotic index and caspase-3 activity. Cells were treated with 0.05, 0.5 and 5 mug/ml of each mycotoxin or with the combinations of two or all three mycotoxins for 24 and 48 h. Changes in LDH and caspase-3 activity, and in apoptotic index showed that the cytotoxic and apoptotic effects of these mycotoxins were concentration- and time- dependent. Significant increase of LDH activity was observed after 48 h of exposure to the highest concentration of FB(1) (45%), BEA (84%) and OTA (77%), as compared to control. OTA increased caspase-3 activity after 24 h of treatment with 0.5 mug/mL (84%), while BEA (319%) and FB(1) (419%) significantly affected this enzyme activity after 48 h (P < 0.05). Increase of caspase-3 activity preceded significant morphological apoptotic changes, which were detected after 48 h of exposure to a single toxin. Combined treatment with FB(1), BEA and OTA resulted mostly in additive effects on LDH activity, and additive and synergistic effects on caspase-3 activity and apoptotic index.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
006-0061117-1242 - Mikromicete, interakcije toksičnih metabolita-zdravlje i prevencija (Maja Šegvić Klarić, )
006-0061245-0977 - Molekularni mehanizmi patogeneze kronične opstrukcijske bolesti pluća (Tihana Žanić-Grubišić, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)