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Analysis of multiple loci can increase reliability of detection of fetal Y chromosome DNA in maternal plasma


Wagner, Jasenka; Džijan, Snježana; Pavan-Jukić, Doroteja; Wagner, Jadranka; Lauc, Gordan
Analysis of multiple loci can increase reliability of detection of fetal Y chromosome DNA in maternal plasma // Prenatal Diagnosis, 28 (2008), 5; 412-416 (međunarodna recenzija, članak, znanstveni)


Naslov
Analysis of multiple loci can increase reliability of detection of fetal Y chromosome DNA in maternal plasma

Autori
Wagner, Jasenka ; Džijan, Snježana ; Pavan-Jukić, Doroteja ; Wagner, Jadranka ; Lauc, Gordan

Izvornik
Prenatal Diagnosis (0197-3851) 28 (2008), 5; 412-416

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
prenatal gender determination; cell-free fetal DNA; Y chromosome DNA; polymorphic STR loci

Sažetak
Aiming to develop more reliable methods for determination of fetal gender from maternal plasma we compared three different systems of PCR detection of Y-chromosome DNA. Cell free DNA was isolated from 96 samples of maternal plasma and a) amplified using AmpFLSTR-Identifiler (15 autosomal STR loci and amelogenin) or AmpFLSTR-Yfiler (16 Y-chromosome STR loci) kits and subsequently analyzed on ABI-PRISM 310 Genetic-Analyzer, or b) analyzed using Quantifiler-Y DNA-Quantification kit. Gender of fetuses was confirmed by cytogenetic analysis or phenotypically at birth. AmpFLSTR-Identifiler and Quantifiler-Y Human Quantification kits were rather reliable in determining fetal gender (95.6% and 98.9%, respectively), but false negatives were still present in both systems. AmpFLSTR Yfiler was found to be fully reliable as it amplified Y-chromosome in all cases of male fetuses, and was thus 100% correct in determining fetal gender. In addition it enabled comparison of polymorphic Y-chromosome loci between father and a child, thus further supporting specificity of obtained results.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
309-0061194-2023 - Glikoproteomika stresa i bolesti povezanih sa stresom (Gordan Lauc, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Osijek,
GENOS d.o.o.

Profili:

Avatar Url Gordan Lauc (autor)

Avatar Url Snježana Džijan (autor)

Avatar Url Jadranka Wagner (autor)

Avatar Url Jasenka Wagner (autor)

Citiraj ovu publikaciju

Wagner, Jasenka; Džijan, Snježana; Pavan-Jukić, Doroteja; Wagner, Jadranka; Lauc, Gordan
Analysis of multiple loci can increase reliability of detection of fetal Y chromosome DNA in maternal plasma // Prenatal Diagnosis, 28 (2008), 5; 412-416 (međunarodna recenzija, članak, znanstveni)
Wagner, J., Džijan, S., Pavan-Jukić, D., Wagner, J. & Lauc, G. (2008) Analysis of multiple loci can increase reliability of detection of fetal Y chromosome DNA in maternal plasma. Prenatal Diagnosis, 28 (5), 412-416.
@article{article, year = {2008}, pages = {412-416}, keywords = {prenatal gender determination, cell-free fetal DNA, Y chromosome DNA, polymorphic STR loci}, journal = {Prenatal Diagnosis}, volume = {28}, number = {5}, issn = {0197-3851}, title = {Analysis of multiple loci can increase reliability of detection of fetal Y chromosome DNA in maternal plasma}, keyword = {prenatal gender determination, cell-free fetal DNA, Y chromosome DNA, polymorphic STR loci} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE