engleski

Thyroid Fine-Needle Aspiration Samples Inadequate for Reverse Transcriptase PCR Analysis

Background. Analysis of different tumor markers by reverse transcriptase polymerase chain reaction (RT-PCR) in fine-needle aspiration samples of thyroid nodules has been studied with the aim to improve the accuracy of preoperative diagnosis of thyroid lesions. The aim of this study was to investigate inadequate thyroid fine-needle aspiration samples for RT-PCR analysis and to determine if there is a correlation between their proportion and method of sampling or diameter of nodules. Methods. A total of 350 fine-needle aspiration samples from patients with thyroid nodules were analyzed. After the aspirate was smeared for conventional cytology the leftover material in the needle was used for RT-PCR analysis in one group of 175 patients. In another group of 175 patients separate puncture was performed to obtain material only for RT-PCR analysis. Samples were considered adequate for RT-PCR analysis if the expression of both glyceraldehyde-3-phosphate dehydrogenase and thyroglobulin was found by RT-PCR. Results. In total, 61 (17.4%) inadequate samples for RT-PCR were detected. All 12 samples that were inadequate for cytological diagnosis were also inadequate for RT-PCR analysis. Proportion of inadequate samples for RT-PCR was significantly higher in samples form leftover material in the needle (21.7%) then in samples from separate puncture (13.1%) (p=0.049). No statistically significant correlation between the adequacy of samples for RT-PCR and the largest diameter of the nodule was found. Conclusion. Proportion of inadequate samples for RT-PCR was higher in samples from leftover material in the needle then in samples from separate puncture.

biopsy; fine-needle; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating); reverse transcriptase polymerase chain reaction; thyroglobulin; thyroid neoplasms; thyroid nodules

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