engleski

Application of Comet assay on human lymphocytes treated with atorvastatin in vitro

This study investigated the genotoxic potential of a therapeutic concentration of atorvastatin, the first-line therapy for reducing low-density lipoprotein (LDL)levels in patients at high risk for atherosclerotic cardiovascular disease in human peripheral blood lymphocytes using the single cell gel electrophoresis (SCGE) - Comet assay.Lymphocyte cultures were treated in vitro with a single drug at a concentration of 30.21 ng/ml. For the Comet assay, cells exposed to atorvastatin for 24, 48 and 72 h were embedded in agarose slides, lysed with alkaline lysis solution and exposed to an electric field. DNA migrated within the agarose and formed comets whose lenght depends on the amount of DNA damage.The Comet assay showed increased DNA damage caused in atorvastatin-exposed human lymphocytes than in corresponding control cells for exposue times of 24, 48 and 72h for the tail lenght and for 72 h for the tail moment. The results obtained in this study point to the significance of biological indicators providing information on the primary genome damage after long-term exposure, which can help to establish drug therapeutic concentrations that do not put patients with high blood cholesterol to a greater treatment-related risk.

comet assay; atorvastatin; human lymphocytes

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