engleski

Induction of embryogenic callus in Cucurbita pepo hypocotyl explants by indole-3-ethanol and its sugar conjugates

Hypocotyl explants of pumpkin (Cucurbita pepo L.) were inoculated aseptically on solid media containing Murashige and Skoog (MS) macro- and micronutrients, organic supplements, glucose as a carbon source, and indoleacetic acid (IAA) or one of its possible metabolic precursors, i. e. indole-3-ethanol [3-(2-hydroxyethyl)indole, tryptophol], 2-(indol-3-yl)ethyl alpha-L-arabinopyranoside (tryptophol arabinoside), and 2-(indol-3-yl)ethyl beta-D-glucopyranoside (tryptophol glucoside). Embryogenic callus and adventitious roots developed on the explants, the response depending on the source of auxin, its concentration, and endogenous, possibly genetic, factors. Hypocotyl sections did not form embryogenic tissues and did not survive on media which did not contain an auxin or auxin precursor. Fewer explants responded to IAA than to indole-3-ethanol. Its arabinopyranoside was even more effective, while only a few hypocotyl sections proliferated in the presence of the corresponding glucopyranoside. Embryogenic callus induced by any of the four above compounds could be subcultured on media containing the same source of auxin. Selected callus lines have been maintained for more than three years and have continued to form embryoids. Indole-3-ethanol and IAA were suitable for clonal propagation of regenerated plantlets by axillary and apical bud culture.

somatic embryogenesis; pumpkin; Cucurbitaceae; auxin biogenesis; indole-3-acetic acid; IAA; tryptophol; tryptophol glucoside; tryptophol arabinoside

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