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Pregled bibliografske jedinice broj: 324399

High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples


Cvek, Josipa; Medić-Šarić, Marica; Jasprica, Ivona; Mornar, Ana
High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples // JPC-Journal of Planar Chromatography-Modern TLC, 20 (2007), 6; 429-435 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 324399 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples

Autori
Cvek, Josipa ; Medić-Šarić, Marica ; Jasprica, Ivona ; Mornar, Ana

Izvornik
JPC-Journal of Planar Chromatography-Modern TLC (0933-4173) 20 (2007), 6; 429-435

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
densitometry; flavonoids; HPTLC; phenolic acids; propolis

Sažetak
Propolis is the generic name for the resinous substance collected by honeybees (Apis mellifera L.) from a variety of plant sources. Because of its popularity in folk medicine, it has become the subject of intense pharmacological and chemical studies over the last 30 years. Increased use of propolis preparations necessitates analysis of its composition and identification of its pharmacologically active constituents. In this paper a simple, selective, and sensitive HPTLC method is reported for determination and quantification of three phenolic acids (caffeic acid, p-coumaric acid, and isoferulic acid) and four flavonoids (pinocembrin, pinocembrin-7-methyl ether, chrysin, and tectochrysin) in seven propolis samples collected from different parts of Croatia. Chromatographic analysis was performed on 20 cm x 10 cm glass-backed silica gel F-254s HPTLC plates with chloroform-methanol-formic acid, 44:3.5:2.5 (v/v) as mobile phase. Quantification was performed by scanning densitometry at the UV absorption maximum of each compound. The method was found to be simple, reliable, and convenient for analysis of these phenolic compounds in Croatian propolis samples. Its analytical performance (precision, accuracy and robustness) fulfilled the acceptance criteria established for TLC methods in the official literature. The method was also shown to be selective and linear.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Projekti:
006-0061117-1237 - Biološki aktivni spojevi, metaboliti i QSAR (Medić-Šarić, Marica, MZOS ) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb


Citiraj ovu publikaciju:

Cvek, Josipa; Medić-Šarić, Marica; Jasprica, Ivona; Mornar, Ana
High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples // JPC-Journal of Planar Chromatography-Modern TLC, 20 (2007), 6; 429-435 (međunarodna recenzija, članak, znanstveni)
Cvek, J., Medić-Šarić, M., Jasprica, I. & Mornar, A. (2007) High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples. JPC-Journal of Planar Chromatography-Modern TLC, 20 (6), 429-435.
@article{article, author = {Cvek, Josipa and Medi\'{c}-\v{S}ari\'{c}, Marica and Jasprica, Ivona and Mornar, Ana}, year = {2007}, pages = {429-435}, keywords = {densitometry, flavonoids, HPTLC, phenolic acids, propolis}, journal = {JPC-Journal of Planar Chromatography-Modern TLC}, volume = {20}, number = {6}, issn = {0933-4173}, title = {High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples}, keyword = {densitometry, flavonoids, HPTLC, phenolic acids, propolis} }
@article{article, author = {Cvek, Josipa and Medi\'{c}-\v{S}ari\'{c}, Marica and Jasprica, Ivona and Mornar, Ana}, year = {2007}, pages = {429-435}, keywords = {densitometry, flavonoids, HPTLC, phenolic acids, propolis}, journal = {JPC-Journal of Planar Chromatography-Modern TLC}, volume = {20}, number = {6}, issn = {0933-4173}, title = {High-performance thin-layer chromatographic analysis of the phenolic acid and flavonoid content of Croatian propolis samples}, keyword = {densitometry, flavonoids, HPTLC, phenolic acids, propolis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





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