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Pregled bibliografske jedinice broj: 324396

Evaluation of antioxidative activity of Croatian propolis samples using DPPH center dot and ABTS(center dot+) stable free radical assays


Jasprica, Ivona; Bojić, Mirza; Mornar, Ana; Bešić, Erim; Bućan, Kajo; Medić-Šarić, Marica
Evaluation of antioxidative activity of Croatian propolis samples using DPPH center dot and ABTS(center dot+) stable free radical assays // Molecules, 12 (2007), 5; 1006-1021 (međunarodna recenzija, članak, znanstveni)


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Naslov
Evaluation of antioxidative activity of Croatian propolis samples using DPPH center dot and ABTS(center dot+) stable free radical assays

Autori
Jasprica, Ivona ; Bojić, Mirza ; Mornar, Ana ; Bešić, Erim ; Bućan, Kajo ; Medić-Šarić, Marica

Izvornik
Molecules (1420-3049) 12 (2007), 5; 1006-1021

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Propolis; DPPH·; ABTS· +; mathematical model; antioxidative efficiency

Sažetak
Propolis is one of the richest sources of plant phenolics (flavonoids and phenolic acids), which are widely recognized as rather strong antioxidants. The aim of our work was to use colored stable free radical (DPPH· and ABTS· +) spectrophotometric and thin-layer chromatographic (TLC) assays to study the antioxidative behavior of the phenolics (caffeic acid, galangin and pinocembrin) most commonly present in Croatian propolis samples obtained from different Croatian regions. We propose a mathematical model providing a more sophisticated interpretation of the obtained results and a new parameter named antioxidative efficiency (AOE) is introduced. The kinetic behaviour of chosen standards determined by spectrophotometric assays follows the exponential decrease of the absorption curve. Explained numerically, AOE represents the absolute value of the first derivative of an absorbance curve in the point A0/e (where A0 is the absorbance measured at t = 0 and e is the natural logarithm base). The advantage of this newly introduced parameter is that it provides an easy and accurate mutual comparison between the rates of antioxidative efficiency of different propolis samples. A TLC assay was only applicable in the case of the DPPH· radical. Dose-response curves were described using a linear function with AOE expressed as a coefficient of the slope. The chromatographic method was shown to be very rapid, reliable and easy-to-perform.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Projekt / tema
006-0061117-1237 - Biološki aktivni spojevi, metaboliti i QSAR (Marica Medić-Šarić, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
KBC Split

Citiraj ovu publikaciju

Jasprica, Ivona; Bojić, Mirza; Mornar, Ana; Bešić, Erim; Bućan, Kajo; Medić-Šarić, Marica
Evaluation of antioxidative activity of Croatian propolis samples using DPPH center dot and ABTS(center dot+) stable free radical assays // Molecules, 12 (2007), 5; 1006-1021 (međunarodna recenzija, članak, znanstveni)
Jasprica, I., Bojić, M., Mornar, A., Bešić, E., Bućan, K. & Medić-Šarić, M. (2007) Evaluation of antioxidative activity of Croatian propolis samples using DPPH center dot and ABTS(center dot+) stable free radical assays. Molecules, 12 (5), 1006-1021.
@article{article, year = {2007}, pages = {1006-1021}, keywords = {Propolis, DPPH and \#903, , ABTS and \#903, +, mathematical model, antioxidative efficiency}, journal = {Molecules}, volume = {12}, number = {5}, issn = {1420-3049}, title = {Evaluation of antioxidative activity of Croatian propolis samples using DPPH center dot and ABTS(center dot+) stable free radical assays}, keyword = {Propolis, DPPH and \#903, , ABTS and \#903, +, mathematical model, antioxidative efficiency} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE