Napredna pretraga

Pregled bibliografske jedinice broj: 323717

Biological effects of three newly synthesized compounds on the cell cultures from the patients affected by Dupuytren’ s contracture


Bratulić, Siniša; Jurišić, Davor; Bujak, Maro; Karminski-Zamola, Grace; Žinić, Biserka; Pavelić, Krešimir; Kraljević, Sandra
Biological effects of three newly synthesized compounds on the cell cultures from the patients affected by Dupuytren’ s contracture // Book of abstracts of the Central European Scientists Meeting
Cavtat, Hrvatska, 2006. (poster, sažetak, znanstveni)


Naslov
Biological effects of three newly synthesized compounds on the cell cultures from the patients affected by Dupuytren’ s contracture

Autori
Bratulić, Siniša ; Jurišić, Davor ; Bujak, Maro ; Karminski-Zamola, Grace ; Žinić, Biserka ; Pavelić, Krešimir ; Kraljević, Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the Central European Scientists Meeting / - , 2006

Skup
EMBO/HHMI Central European Scientists Meeting

Mjesto i datum
Cavtat, Hrvatska, 15.- 17.06. 2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Neobjavljeni rad

Ključne riječi
Dupuytrenova kontrakture; antiproliferativni učinak; primarne stanične kulture
(Dupuytren's contracture; antiproliferative effect; primary cell cultures)

Sažetak
Dupuytren’ s contracture (DC) is a palmar fibromatosis having an obscure etiology. It is characterized by nodular fibroblastic proliferation and accumulation of myofibroblasts. So far, the only successful treatment was surgical excision or incision of diseased fascia, often accompanied by high recurrence rates. Experimental chemotherapy, such as 5-fluorouracil (5-FU), proved to be useful in acting locally in the palm by inhibiting myofibroblasts proliferation. 5-FU was shown to inhibit both, proliferation and myofibroblast differentiation in DC’ s cell cultures. We have therefore established primary cell cultures as a model for studying the effects of 3 newly synthesized chemical compounds on proliferation and differentiation of Dupuytren’ s fibroblasts/myofibrobalsts. Subsequently, cells were characterized by several proliferation assays and the cell cycle distribution was determined using flow cytometry.We showed cells from the affected part (D) of fascia to proliferate faster then the cells from non-affected part (ND). Also, the percentage of cells in the S-phase of the cell cycle was higher in the D than in the ND cells. The presence of myofibroblasts in culture was ascertained by immunocytochemistry and Western blot method, as we assed the quantity of α -smooth actin as a molecular marker for differentiated fibroblasts. We have further tested cytotoxic and antiproliferatve effects of 3 compounds (a N-sulfonylpyrimidine derivative, an amidino substituted benzimidazo(1, 2-a)quinoline derivative and an amido-substituted dithieno-kinolone derivative) by MTT-test and showed them all to have a marked antiproliferative effect on established cell lines. Their mechanisms of action were further studied in parallel experiments by cell cycle analyses, immunocitochemistry and Western blots. Obtained results implicated that tested compounds exerted different but rather specific effects related to the progression through the cell cycle and differentiation of fibroblasts into myofibroblasts.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0125005
14V09809

Ustanove
Medicinski fakultet, Rijeka,
Institut "Ruđer Bošković", Zagreb