Manual solid-phase peptide synthesis of metallocene - peptide bioconjugates (CROSBI ID 136777)
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Podaci o odgovornosti
Kirin, Srećko I. ; Noor, Fozia ; Metzler-Nolte, Nils ; Mier, Walter
engleski
Manual solid-phase peptide synthesis of metallocene - peptide bioconjugates
We describe a simple procedure for the manual solid-phase synthesis of peptides. No expensive or specialized equipment is needed. Moreover, a variety of markers can be attached to the N-terminus. We describe two organometallic markers and a fluroescent dye, in addition to an unlabeled derivative that serves as a comparison. The experiment described herein was successfully carried out by fourth-year undergraduate students of molecular biotechnology. By carrying out this experiment, the students gained first-hand experience for one of the most important modern synthesis techniques.
chemical education; solid phase peptide synthesis; ferrocene
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