engleski

Switch in ovarian muscarinic receptor signaling after hemicastration

It seems that hemicastration induces functional changes of ovarian muscarinic receptors [Can J Physiol Pharm 1994 ; 72(Suppl 1):379]. In the present study, 1, 2-diacylglycerol (DAG) levels in the remnant ovaries were, as measured 15 minutes following the operation, increased (by 2-3 fold), especially (by 3-5 fold) if organs were incubated with carbachol (50 mM). The hemicastration-induced increase was inhibited by atropine (iv) or botulinum toxin (into ovarian bursa) pretreatments, or by abdominal vagotomy. The effect of carbachol was inhibited by atropine, or by abdominal vagotomy performed prior to hemicastration, but was preserved if vagotomy was performed 10 minutes after hemicastration. Carbachol, atropine or botulinum toxin had no effects on DAG levels in the ovaries of sham-operated rats. The results suggest that hemicastration induces a) DAG accumulation in the remnant ovary via innervated muscarinic receptors ; b) immediate coupling of the muscarinic receptors to DAG signaling for which a vagus-dependent information is needed.

muscarinic receptor; signaling; ovary; compensatory growth; mitogen

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