Introduction of an internal dipeptide into PNA oligomers: Thermal melting studies and further functionalization (CROSBI ID 136713)
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Kersebohm, Tim ; Kirin, Srećko I. ; Metzler-Nolte, Nils
engleski
Introduction of an internal dipeptide into PNA oligomers: Thermal melting studies and further functionalization
The solid phase synthesis of PNA oligomers with the internal dipeptide Gly-Phe is presented and the interaction with complementary DNA investigated. UV absorbance melting experiments with different but complementary DNA sequences show that stable PNAradical dotDNA duplexes are only obtained when there is no DNA base opposite the dipeptide unit. Instead, the dipeptide spacer forms a loop-like structure within the duplex. Further functionalization with N-heterocyclic ligands is described. p-Nitro-phenylalanine is introduced in place of Phe during solid phase synthesis and subsequently reduced to p-amino-phenylalanine. Reaction with activated acids provides the ligand conjugates in high yield and purity. This strategy opens a universal route to a large number of internal substitutions in PNA chemistry.
artificial nucleases; bis(2-picolyl) amine; peptide nucleic acid (PNA); solid phase peptide synthesis; terpyridine
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