engleski

Genetic changes of CDH1, APC and CTNNB1 found in human brain tumors

Research carried out in this paper focused on changes of genes, E-cadherin (CDH1), adenomatous polyposis coli (APC) and beta-catenin (CTNNB1) in a palette of 50 central nervous system tumors. All gene products are components of adherens junctions, but are also involved in Wnt signalling. The results of our analysis showed LOH of CDH1 gene in 31% of meningiomas examined (correlation significant at 0.002 level). One LOH was found in a single case of germinoma, while other tumor types did not demonstrate changes of the CDH1. Fourteen samples (29.2%) with changes of APC gene were observed. The changes were distributed to: 33.3% of glioblastomas, 27% of meningiomas, 1 LOH in five informative astocytomas (20%), and 1 in six informative neurinomas (17%). One oligoastrocytoma showed LOH at exon 11 and one medulloblastoma had allelic imbalance at both exons. Five samples (10%) showed heteroduplexes in β -catenin’ s exon 3. Potential mutations were confined to two meningiomas, an astrocytoma, a glioblastoma, and a germinoma. Our results suggest that genetic changes of wnt components are involved in brain tumor genesis. Changes of E-cadherin are involved in meningiomas, while changes of APC gene are distributed among different tumor types, with glioblastomas showing the highest percentage.

adenomatous polyposis coli gene (APC); beta-catenin gene (CTNNB1); E-cadherin gene (CDH1); tumors of the CNS; wnt signaling pathway

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