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Correlation of two methods for determination of cathepsin D in breast carcinoma (immunochemistry and ELISA in cytosol)

We have studied the correlation of two methods(immunohistology and ELISA in cytosol) of cathepsin D(CD) determination in breast carcinoma patients. Fifty sixspecimens of tumor tissue were collected consecutively, andCD expression in tumor tissue and tissue macrophageswas determined by standard immunohistochemistry using the aNCL-CDmanti-cathepsin D mouse monoclonal antibody (Novocastra Laboratories Ltd., Newcastle, UK). Additionally, CD concentration was determined byELISA in cytosol of the same breast carcinomaspecimens. CD positivity was correlated with tumor size, histological grade of tumor, and the cytosol progesteroneand estrogen receptor concentrations. There was no statisticallysignificant correlation between examined parameters and either CDpositivity by immunohistochemistry or cytosol CD concentration. Thecorrelation between CD expression in tumor cells ofbreast carcinoma by immunohistochemistry and cytosol CD positivitywas not found either. However, there was asignificant association between abundance of CD positive stromalmacrophages and cytosol CD concentration in all histologicaltumor types (p < 0.05). CD positive macrophageswere abundant in most of cytosol CD positivespecimens.These results suggest that breast cancer cytosol CDconcentration is the cumulative result of CD contentin both carcinoma cells and stromal macrophages.

breast carcinoma; cathepsin D; ELISA in cytosol; immunohistochemistry

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