Isolation of mussel metallothionein as biomarker of metal exposure (CROSBI ID 532234)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Raspor, Biserka ; Erk, Marijana
engleski
Isolation of mussel metallothionein as biomarker of metal exposure
Lysis of cells is accomplished using a motorised glass/teflon homogeniser. During the homogenizing procedure the samples should be kept constantly cooled (on ice) and all buffers added should be cold. Beta-mercaptoethanol is added to protect protein thiols from oxidation. The supernatant obtained after centrifugation at 50000 x g for 120 min is called the S50-fraction and contains the cytosol.
metallothionein; biochemical isolation
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
26-27-x.
2007.
objavljeno
Podaci o matičnoj publikaciji
Marine Chemistry - Analytical methods, trace elements and organic matter in marine environment
Plavšić, Marta
Zagreb: Institut Ruđer Bošković
978-953-6690-71-8
Podaci o skupu
Marine Science and Coastal Management in the Adriatic, Western Balkans ; Course 3
ostalo
12.11.2007-17.11.2007
Zagreb, Hrvatska
