Trichomonas tenax proteolytic activity (CROSBI ID 84468)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Šegović, Sanja ; Buntak-Kobler Dunja ; Galić, Nada ; Katunarić, Marina
engleski
Trichomonas tenax proteolytic activity
In this study, proteolytic activity of Trichomonas tenax collected directly from patient's dentobacterial plaque was examined. Electrophoretic method involving polyacrylamide gels (Commassie Brilliant Blue R-250) and electrophoretic method involving gelatin- containing polyacrylamide gels, have been used to analyse Trichomonas tenax proteolytic activity. The most obvious and the fastest activities were obtained when gels were incubated in pH 4.6 ; followed by results of incubating in pH 5.6 ; while in pH 2.8 activity was less effective but still obvious. Proteolytic activities were the most effective in area of protein MW 36 kDa. Different activities of enzymes depending on pH of incubated media indicate the presence of different endopeptidase in cell lysates of protozoon Trichomonas tenax from dentobacterial plaque.
trichomonas tenax proteolytic activity ; dentobacterial plaque ; polyacrylamide gels
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