engleski

The expression of bone morphogenetic protein-7 and its receptors in the human normal kidney and kidney cancer

Bone morphogenetic protein-7 has an important role during fetal kidney development. In adult human kidney the expression was found in proximal tubule and glomerular podocytes. The function of BMP-7 in normal kidney is still unclear but the reviews revealed protective role of BMP-7 in acute and chronic kidney disease. BMP-7 is efficient as antifibrotic factor and is able to reverse epithelial-mesenchymal transformation. The aim of the study was to explore the expression of BMP-7 in human normal kidney and kidney cancer cells. Immunohistochemical staining with anti BMP-7, anti BMPR-IA, anti BMPR-IB and anti BMPR-II was performed. Slides were incubated with the primary antibodies overnight at 4°C in PBS. The expression of BMP-7 was found in epithelial cells of distal tubule. But the expression of related BMPs receptors was found in epithelial cells od fistal and proximal tubules. Among receptors BMPR-IB expression was very strong. In the tissue of kidney cancer the expression of BMP-7 was negative. In the borderline tissue of the kidney cancer the expression of BMP-7 was still present. In the kidney cancer cells the expression of BMPs receptors was present especially BMPR-IA and BMPR-IB while BMPR-II was less expressed.

bone morphogenetic protein-7; BMP-receptors; kidney; kidney cancer; immunohistochemistry

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