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Analysis of clonality in T-lymphoprolifertive diseases by multiplex PCR


Zadro, Renata; Kralj, Danijela; Aurer, Igor; Kovačević-Metelko, Jasminka; Labar, Boris; Stavljenić-Rukavina, Ana
Analysis of clonality in T-lymphoprolifertive diseases by multiplex PCR // Abstracts of The Second Croatian Congress in Human Genetics ; u: Paediatria Croatica 42 (1998) (S3) / Stavljenić-Rukavina, Ana (ur.).
Zagreb: Klinika za pedijatriju, 1998. str. 40-40 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Analysis of clonality in T-lymphoprolifertive diseases by multiplex PCR

Autori
Zadro, Renata ; Kralj, Danijela ; Aurer, Igor ; Kovačević-Metelko, Jasminka ; Labar, Boris ; Stavljenić-Rukavina, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of The Second Croatian Congress in Human Genetics ; u: Paediatria Croatica 42 (1998) (S3) / Stavljenić-Rukavina, Ana - Zagreb : Klinika za pedijatriju, 1998, 40-40

Skup
Croatian Congress in Human Genetics (2 ; 1998)

Mjesto i datum
Zagreb, Hrvatska, 21.-24.10.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
PCR; TCR-gamma; acute leukemia; diagnosis

Sažetak
Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix the patient specific T-cell receptor gamma-rearrangement was determined: five of ten patients showed the exclusive presence of a single T-cell receptor gamma-gene rearrangement. Three patients exhibited two rearranged TCR-gamma genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segment. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represnets a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
214006

Ustanove
Klinički bolnički centar Zagreb

Časopis indeksira:


  • Scopus