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Detection of TCR-gamma gene rearrangement in acute lymphoblastic leukemia by multiplex PCR


Selak, Nives; Zadro, Renata; Aurer, Igor; Mrsić, Mirando; Labar, Boris; Stavljenić-Rukavina, Ana
Detection of TCR-gamma gene rearrangement in acute lymphoblastic leukemia by multiplex PCR // V. seminar New Trends in the Treatment of Acute Leukemia / Mrsić, Mirando ; Labar, Boris (ur.).
Zagreb, Hrvatska: University Hospital Rebro Zagreb and European School of Oncology, 1998. str. 21-21 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Detection of TCR-gamma gene rearrangement in acute lymphoblastic leukemia by multiplex PCR

Autori
Selak, Nives ; Zadro, Renata ; Aurer, Igor ; Mrsić, Mirando ; Labar, Boris ; Stavljenić-Rukavina, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
V. seminar New Trends in the Treatment of Acute Leukemia / Mrsić, Mirando ; Labar, Boris - Zagreb, Hrvatska : University Hospital Rebro Zagreb and European School of Oncology, 1998, 21-21

Skup
V. seminar New Trends in the Treatment of Acute Leukemia

Mjesto i datum
Dubrovnik, Hrvatska, 06-09.09.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
PCR; TCR-gamma; acute leukemia

Sažetak
Detection of clonal antigen receptor gene rearrangement can be used to determine lineage of lymphoproliferation. As in T-cell malignancies there is no immunopathological equivalent to monoclonal surface immunoglobulin like in B-cell neoplasms, molecular genetic studies are especially important. In our study we have tested a series of patients with the diagnosis of acute lymphoblastic leukemia (ALL) as well as normal individuals for the T-cell receptor gamma-chain gene rearrangement. Genomic DNA was isolated from peripheral blood or bone marrow of 20 ALL patients as well as from peripheral blood of 20 healthy individuals according to standard procedures. Four out of seven patients showed the exclusive presence of a single TCR-gamma gene rearrangement. Two patients exhibited two rearranged TCR-gamma genes, while in one patient positive reaction was obtained with three VJ primer combinations producing only two bands on agarose gels. Molecular analysis of rearranged T-cell receptor genes by multiplex PCR represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
214006

Ustanove
Klinički bolnički centar Zagreb