engleski

Detection of TCR-gamma gene rearrangement in acute lymphoblastic leukemia by multiplex PCR

Detection of clonal antigen receptor gene rearrangement can be used to determine lineage of lymphoproliferation. As in T-cell malignancies there is no immunopathological equivalent to monoclonal surface immunoglobulin like in B-cell neoplasms, molecular genetic studies are especially important. In our study we have tested a series of patients with the diagnosis of acute lymphoblastic leukemia (ALL) as well as normal individuals for the T-cell receptor gamma-chain gene rearrangement. Genomic DNA was isolated from peripheral blood or bone marrow of 20 ALL patients as well as from peripheral blood of 20 healthy individuals according to standard procedures. Four out of seven patients showed the exclusive presence of a single TCR-gamma gene rearrangement. Two patients exhibited two rearranged TCR-gamma genes, while in one patient positive reaction was obtained with three VJ primer combinations producing only two bands on agarose gels. Molecular analysis of rearranged T-cell receptor genes by multiplex PCR represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

PCR; TCR-gamma; acute leukemia

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