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Pregled bibliografske jedinice broj: 312040

Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses


Korolija, Marina; Hadžija, Mirko; Pavković, Pajica; Kapitanović, Sanja; Popović Hadžija, Marijana
Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses // Croatica Chemica Acta, 81 (2008), 3; 437-441 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 312040 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses

Autori
Korolija, Marina ; Hadžija, Mirko ; Pavković, Pajica ; Kapitanović, Sanja ; Popović Hadžija, Marijana

Izvornik
Croatica Chemica Acta (0011-1643) 81 (2008), 3; 437-441

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DPC4; Loss of heterozygosity; allele; Spreadex polymer NAB

Sažetak
The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue were tested. Elctrophoresis of amplified loci was performed on a standard acrylamide / N, N methylene-bis-acrylamide gel, and the same gel containing Spreadex Polymer, Native Acrylamide-Bis. Standard gel displayed 36 samples of normal tissue as homozygous. Electrophoresis of the same samples on gel with addition of polymer revealed 12/36 heterozygous samples. Five matched tumor samples (of 12) displayed loss of heterozygosity what could not be detected on standard gel, because of its lower resolving power. Electrophoresis on a new polymer presents a simple, inexpensive, reproducible and non-radioactive method for loss of heterozygosity detection, with improved performances (increased sensitivity, less sample needed and full separation of closely spaced bands). Application of the polymer may contribute to a vide variety of diagnostic procedures.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
098-0982464-2460 - Dobivanje struktura nalik Langerhansovim otočićima iz matičnih stanica miša (Hadžija, Mirko, MZOS ) ( POIROT)
098-0982464-2508 - Molekularna genetika i farmakogenetika gastrointestinalnih tumora (Kapitanović, Sanja, MZOS ) ( POIROT)

Ustanove:
Klinika za dijabetes, endokrinologiju i bolesti metabolizma Vuk Vrhovac,
Institut "Ruđer Bošković", Zagreb


Citiraj ovu publikaciju

Korolija, Marina; Hadžija, Mirko; Pavković, Pajica; Kapitanović, Sanja; Popović Hadžija, Marijana
Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses // Croatica Chemica Acta, 81 (2008), 3; 437-441 (međunarodna recenzija, članak, znanstveni)
Korolija, M., Hadžija, M., Pavković, P., Kapitanović, S. & Popović Hadžija, M. (2008) Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses. Croatica Chemica Acta, 81 (3), 437-441.
@article{article, year = {2008}, pages = {437-441}, keywords = {DPC4, Loss of heterozygosity, allele, Spreadex polymer NAB}, journal = {Croatica Chemica Acta}, volume = {81}, number = {3}, issn = {0011-1643}, title = {Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses}, keyword = {DPC4, Loss of heterozygosity, allele, Spreadex polymer NAB} }
@article{article, year = {2008}, pages = {437-441}, keywords = {DPC4, Loss of heterozygosity, allele, Spreadex polymer NAB}, journal = {Croatica Chemica Acta}, volume = {81}, number = {3}, issn = {0011-1643}, title = {Improved performances of polyacrylamide gel by addition of a new type of polymer: detection of allelic losses}, keyword = {DPC4, Loss of heterozygosity, allele, Spreadex polymer NAB} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





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