Peripheral blood lymphocytes subpopulations and immune response of chicks to inactivated Newcastle disease virus given by nebulization (CROSBI ID 531033)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Ciglar Grozdanić, Irena ; Šver, Lidija ; Čajavec, Stanislav ; Gottstein, Željko ; Židovec-Lepej, Snježana ; Mazija, Hrvoje
engleski
Peripheral blood lymphocytes subpopulations and immune response of chicks to inactivated Newcastle disease virus given by nebulization
Newcastle disease (ND) is the most serious infectious disease of poultry causing substantial losses to the poultry industry. Vaccination programs against ND include the use of live vaccines administrated mainly mass by spray or aerosol, but vaccinal reaction often appears. Inactivated vaccines are applied for revaccination and require individual application. The present study was carried out to examine the possible induction of immune response following immunization of newly hatched chicks with inactivated La Sota NDV vaccine by ultrasonic nebulizer Sonovac® ; 095. Additionally, group of chicks was immunized with the same inactivated antigen in mixture with Parapoxvirus ovis as an immune response modifier (IRM) in order to test its immunostimulating activity. Immune response to inactivated NDV was estimated by HI test in week intervals up to 42nd day of age, and by survival after challenge infection. Nevertheless, inactivated antigen didn’ t induce specific antibodies response, which could be detected by HI test, but chickens remained fully protected after challenges (70-100%). Additionally, the changes of leukocytes subpopulation in peripheral blood were analyzed by means of flow cytometry using specific monoclonal antibodies for chicken CD45, Ia, Bu-1 and IgA cell surface molecules. Significant increase (p<0.05) in B cells subpopulations (phenotypes Bu-1+, Bu-1+Ia+, Bu-1+IgA+) was observed 3 days post immunization in vaccinated groups. Further investigations should include the use of more effective immunomodulator and different (higher) dose of inactivated antigen. Results achieved indicate the possible use of inactivated NDV antigen in specific immunoprotection of newly hatched chicks.
inactivated Newcastle disease virus; immune response; nebulization; lymphocytes
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Podaci o prilogu
62-x.
2007.
objavljeno
Podaci o matičnoj publikaciji
Zagreb: Hrvatsko imunološko društvo
Podaci o skupu
Annual meeting of the Croatian Immunological Society 2007
poster
19.10.2007-21.10.2007
Rovinj, Hrvatska