engleski

Anti-inflammatory properties of early pregnancy decidual antigen presenting cells after the bindnig of natural ligands

The distribution and the function of endocytic receptors CD206 and CD209 were analyzed at maternal-fetal interface. Immunohistology showed overlapping, but not identical localization of CD206 and CD209 in human early pregnancy deciduas. CD206 are found on CD14+ cells around the glands, whereas CD209+ cells were disseminated in the stroma. Both receptors are expressed on decidual CD1a+ cells and CD1a-CD14+ macrophages. In macrophages recovered from the suspension of decidual mononuclear cells which had been cultured with IL-4 (24 hrs), CD206 expression and CD206 mediated FITC-dextran internalization were enhanced. Progesterone, Progesterone Induced Blocking Factor and LPS was inefficient. Tumour associated glycoprotein-72 (TAG-72) and MUC-1 bind to carbohydrate recognition domain of CD206 and CD209 on CD1a+ cells and/or macrophages, as well as mannan (positive control). TAG-72 and MUC-1 block endocytosis of FITC-dextran by CD14+ cells. TAG-72 decreases the percentage of CD80 expressing CD14+ cells and CD80, CD83, CD86 HLA-DR expressing CD1a+ cells, and decreases intracellular production of pro-inflammatory (IFN- , IL-15 or IL-18) cytokines in macrophages and/or CD1a+ cells. In both subpopulations IL-10 expression was enhanced after the stimulation with TAG-72. MUC-1, did not change the phenotype, but it decreased the percentage of IL-15 and IL-18 expressing CD14+ cells. CD206 and CD209 are able to bind endogenous, decidual tissue specific ligands, and lead the antigen presenting cells towards anti-inflammatory activating patterns. Acknowledgement: The experiments are partially financed by the grants of “ EMBIC” European FP6 project No. 512040, LSHM-CT-2004-512040, as well as Croatian Ministry of Science, Education and Sports No. 0376 and No. 0377.

early pregnancy; decidua; TAG-72; inflammation

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